《植物生理学报》 2013, 49(9): 917-922

肥荚红豆种子无菌诱导植株再生及快速繁殖

彭绿春^1,2,3,4, 王丽花^1,2,3,4, 马双喜⁵, 苏艳^1,2,3,4, 张艺萍^1,2,3,4, 宋杰^1,3,4, 瞿素萍^1,2,3,4,*

¹云南省农业科学院花卉研究所, 昆明650205; ²农业部花卉产品质量监督检验测试中心(昆明), 昆明650205; ³云南省花卉育种重点实验室, 昆明650205; ⁴云南花卉技术工程研究中心, 昆明650205; ⁵云南省玉溪市易门县林业局, 云南玉溪651100

通信作者：瞿素萍；E-mail: qusuping@sina.com；Tel: 0871-5895288

摘　要：

以肥荚红豆种子为外植体, 研究了种子预处理、诱导萌发、不定芽增殖、壮苗、生根的方法, 摸索培养基中激素的最佳配比, 建立了肥荚红豆组织培养快速繁殖技术。结果表明: 5%~10% NaOH溶液浸泡种子1 h的预处理有利于促进肥荚红豆种皮的剥离和种子萌发; 适宜于种子萌发的培养基为MS (Read)+6-BA 0.5 mg·L^-1+NAA 0.1 mg·L^-1, 萌发率可达100%; 最佳增殖培养基为Read+6-BA 1.0~1.5 mg·L^-1+KT 0.1 mg·L^-1+NAA 0.1 mg·L^-1, 增殖数可达每瓶23个以上; 最佳壮苗培养基为Read+6-BA 0~0.5 mg·L^-1+NAA 0.1 mg·L^-1; 最佳生根培养基为Read+IAA 1.0 mg·L^-1, 其生根率可达90.54%。研究结果提高了肥荚红豆的繁殖效率, 有效促进肥荚红豆组培规模化育苗技术的成熟与推广应用, 对推动其产业开发应用具有重要意义。

关键词：肥荚红豆; 种子; 植株再生; 高效繁殖

收稿：2013-05-14   修定：2013-06-04

资助：云南省科技计划项目(2011EB111)。

Plant Regeneration through Seed Asptic Germination and Rapid Propagation Technology of Ormosia fordiana Oliv.

PENG Lü-Chun^1,2,3,4, WANG Li-Hua^1,2,3,4, MA Shuang-Xi⁵, SU Yan^1,2,3,4, ZHANG Yi-Ping^1,2,3,4, SONG Jie^1,3,4, QU Su-Ping^1,2,3,4,*

¹Flower Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650205, China; ²Supervision and Testing Centre for Flowers of Ministry of Agriculture (Kunming), Kunming 650205, China; ³Yunnan Flower Breeding Key Laboratory, Kunming 650205, China; ⁴Yunnan Flower Research and Development Center, Kunming 650205, China; ⁵Forestry Bureau of Yimen County, Yuxi, Yunnan 651100, China

Corresponding author: ; E-mail: qusuping@sina.com; Tel: 0871-5895288

Abstract:

The plant regeneration system of Ormosia fordiana Oliv. was studied with the pretreatment and ger-mination induction of seed, multiplication and hardening of adventitious bud, rooting of the shoot. The results showed that the best seed germination medium was MS (Read)+6-BA 0.5 mg·L^-1+NAA 0.1 mg·L^-1 with 100% of germination rate, and the best multiplication medium was Read+6-BA 1.0–1.5 mg·L^-1+KT 0.1 mg·L^-1+NAA 0.1 mg·L^-1 which has more than twenty-three multiplication buds in each bottle. Read+6-BA 0–0.5 mg·L^-1+ NAA 0.1 mg·L^-1 was the best hardening medium. The best rooting medium was Read+IAA 1.0 mg·L^-1 with 90.54% of the rooting rate. The research would promote the reproductive efficiency of Ormosia fordiana Oliv.. It improved the maturity and application of large-scale seedling technology, and had the vital significance on development and application of Ormosia fordiana Oliv..

Key words: Ormosia fordiana Oliv., seed; plant regeneration; effective multiplication